ID   C17 NKX2-1-GFP SFTPC-tdTomato
AC   CVCL_WN83
SY   C17 NKX2-1GFP;SFTPCtdTomato; C17-NGST; C17 NGST; iPS17-ST
DR   Wikidata; Q93439149
RX   PubMed=28965766;
CC   From: Boston University School of Medicine; Boston; USA.
CC   Population: Caucasian.
CC   Characteristics: Using TALEN a eGFP-pA-loxP-PGKp-PuroR-pA-loxP was inserted at the NKX2-1 translational start site and a tdTomato-pA-loxP-PGKp-PuroR-pA-loxP was inserted at the SFTPC translational start site.
CC   Sequence variation: Mutation; HGNC; 1884; CFTR; Simple; p.Ile507del (c.1516_1518ATC[1]) (c.1519_1521delATC); ClinVar=VCV000007106; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1884; CFTR; Simple; p.Phe508del (c.1521_1523delCTT); ClinVar=VCV000007105; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=TALEN; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=TALEN; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Skin; UBERON=UBERON_0002097.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
DI   NCIt; C2975; Cystic fibrosis
DI   ORDO; Orphanet_586; Cystic fibrosis
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D3D6 ! C17 NKX2-1-GFP
SX   Male
AG   17Y
CA   Induced pluripotent stem cell
DT   Created: 24-05-19; Last updated: 10-09-24; Version: 12
//
RX   PubMed=28965766; DOI=10.1016/j.stem.2017.08.014; PMCID=PMC5755620;
RA   Jacob A., Morley M., Hawkins F., McCauley K.B., Jean J.-C., Heins H.,
RA   Na C.-L., Weaver T.E., Vedaie M., Hurley K., Hinds A., Russo S.J.,
RA   Kook S., Zacharias W., Ochs M., Traber K., Quinton L.J., Crane A.M.,
RA   Davis B.R., White F.V., Wambach J., Whitsett J.A., Cole F.S.,
RA   Morrisey E.E., Guttentag S.H., Beers M.F., Kotton D.N.;
RT   "Differentiation of human pluripotent stem cells into functional lung
RT   alveolar epithelial cells.";
RL   Cell Stem Cell 21:472-488.e10(2017).
//