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Cellosaurus HeLa-EM2-11ht (CVCL_WN71)

[Text version]
Cell line name HeLa-EM2-11ht
Synonyms HeLa EM2-11ht
Accession CVCL_WN71
Resource Identification Initiative To cite this cell line use: HeLa-EM2-11ht (RRID:CVCL_WN71)
Comments Population: African American.
Characteristics: Stably expresses an improved version of the reverse tetracycline controlled transactivator (rtTA2S-M2) under the control of the human EF1a promoter. Also express an hygromycin B phosphotransferase-thymidine kinase (HygTk) fusion gene as a positive/negative selection marker.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=19264799; DOI=10.1093/nar/gkp108; PMCID=PMC2673444
Weidenfeld I., Gossen M., Low R., Kentner D., Berger S., Gorlich D., Bartsch D., Bujard H., Schonig K.
Inducible expression of coding and inhibitory RNAs from retargetable genomic loci.
Nucleic Acids Res. 37:e50.1-e50.11(2009)

Cross-references
Encyclopedic resources Wikidata; Q94100654
Entry history
Entry creation24-May-2019
Last entry update10-Sep-2024
Version number8