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Cellosaurus VK219 (CVCL_WL55)

[Text version]
Cell line name VK219
Synonyms VK.219; Vero-rKSHV.219
Accession CVCL_WL55
Resource Identification Initiative To cite this cell line use: VK219 (RRID:CVCL_WL55)
Comments Group: Non-human primate cell line.
Characteristics: rKSHV.219 includes a RFP-GFP-Puro construct that contains RFP under the control of the lytic PAN RNA promoter, GFP under the control of human EF1a promoter and PuroR expressed by the RSV promoter.
Virology: Infected with a recombinant human herpesvirus 8 (HHV-8/KSHV) rKSHV.219.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Kidney, epithelium; UBERON=UBERON_0004819.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Chlorocebus sabaeus (Green monkey) (Simia sabaea) (NCBI Taxonomy: 60711)
Hierarchy Parent: CVCL_0059 (Vero)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=15246263; DOI=10.1016/j.virol.2004.03.049
Vieira J., O'Hearn P.M.
Use of the red fluorescent protein as a marker of Kaposi's sarcoma-associated herpesvirus lytic gene expression.
Virology 325:225-240(2004)

Cross-references
Encyclopedic resources Wikidata; Q98134520
Entry history
Entry creation24-May-2019
Last entry update10-Sep-2024
Version number11