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Cellosaurus WISC014i-SC1 (CVCL_VS05)

[Text version]
Cell line name WISC014i-SC1
Synonyms GFP-CCR5mut-hiPSC-Comb2-SC1
Accession CVCL_VS05
Resource Identification Initiative To cite this cell line use: WISC014i-SC1 (RRID:CVCL_VS05)
Comments From: University of Wisconsin; Madison; USA.
Characteristics: The expression of the Clover GFP gene is under the control of the human EF1a promoter.
Knockout cell: Method=CRISPR/Cas9; HGNC; 1606; CCR5.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 4Z641; Clover (Note=Basic green/yellow fluorescent protein derived from GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
Cell type: Fibroblast of foreskin; CL=CL_1001608.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_VS02 (WISC011i-inGFPpuro)
Sex of cell Male
Age at sampling <1M
Category Induced pluripotent stem cell
Publications

PubMed=26670276; DOI=10.1038/mtna.2015.42
Kang H., Minder P., Park M.A., Mesquitta W.-T., Torbett B.E., Slukvin I.I.
CCR5 disruption in induced pluripotent stem cells using CRISPR/Cas9 provides selective resistance of immune cells to CCR5-tropic HIV-1 virus.
Mol. Ther. Nucleic Acids 4:e268.1-e268.10(2015)

Cross-references
Encyclopedic resources Wikidata; Q98134936
Entry history
Entry creation25-Feb-2019
Last entry update10-Sep-2024
Version number10