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Cellosaurus U2OS#18-XRCC2-5E (CVCL_VR84)

[Text version]
Cell line name U2OS#18-XRCC2-5E
Accession CVCL_VR84
Resource Identification Initiative To cite this cell line use: U2OS#18-XRCC2-5E (RRID:CVCL_VR84)
Comments Population: Caucasian.
Characteristics: Contains a 'nested intron' reporter construct HRsub that allows to study sister chromatid recombination (SCR) by positive selection of 'long tract' gene conversion events between sister chromatids.
Doubling time: ~4-6 days (DSMZ=ACC-836).
Knockout cell: Method=CRISPR/Cas9; HGNC; 12829; XRCC2.
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_VR75 (U2OS#18)
Children:
CVCL_VR85 (U2OS#18-XRCC2-5E/X2+)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-836

Markers:
AmelogeninX
CSF1PO12,13
D2S133820,24
D3S135816
D5S8188,11
D7S82011,12
D8S117912,14
D13S31713
D16S53911,12
D18S5112,14
D19S43315
D21S1131,32
FGA20
Penta D9
Penta E10,13
TH016,9.3
TPOX12
vWA14,18

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Cross-references
Cell line collections (Providers) DSMZ; ACC-836
Cell line databases/resources DSMZCellDive; ACC-836
Encyclopedic resources Wikidata; Q98133783
Entry history
Entry creation25-Feb-2019
Last entry update10-Sep-2024
Version number11