ID   U2OS#18-RAD51B-8/B+
AC   CVCL_VR79
DR   DSMZ; ACC-838
DR   DSMZCellDive; ACC-838
DR   Wikidata; Q98133778
CC   Population: Caucasian.
CC   Characteristics: The parental RAD51B KO cell line is functionally reconstituted by retroviral transfection of RAD51B.
CC   Characteristics: Contains a 'nested intron' reporter construct HRsub that allows to study sister chromatid recombination (SCR) by positive selection of 'long tract' gene conversion events between sister chromatids.
CC   Doubling time: ~25-30 hours (DSMZ=ACC-838).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:9822; RAD51B.
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:9822; RAD51B.
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
ST   Source(s): DSMZ=ACC-838
ST   Amelogenin: X
ST   CSF1PO: 13
ST   D13S317: 13
ST   D16S539: 11,12
ST   D18S51: 12,14
ST   D19S433: 15
ST   D21S11: 31,32
ST   D2S1338: 20,24
ST   D3S1358: 16
ST   D5S818: 11
ST   D7S820: 11,12
ST   D8S1179: 12,14
ST   FGA: 20
ST   Penta D: 9
ST   Penta E: 10,13
ST   TH01: 6,9.3
ST   TPOX: 11,12
ST   vWA: 14,18
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_VR78 ! U2OS#18-RAD51B-8
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 25-02-19; Last updated: 19-12-24; Version: 13
//