Cellosaurus A549 dCas9-KRAB (CVCL_VR73)
| Cell line name | A549 dCas9-KRAB | ||||||||||||||||||
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| Accession | CVCL_VR73 | ||||||||||||||||||
| Resource Identification Initiative | To cite this cell line use: A549 dCas9-KRAB (RRID:CVCL_VR73) | ||||||||||||||||||
| Comments | Population: Caucasian. Characteristics: Using CRISPR/Cas9 a construct containing KRAB-dCas9-2A-Pac-2A-RFP-PA was introduced in the AAVS1 safe harbor locus. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Lung; UBERON=UBERON_0002048. | ||||||||||||||||||
| Sequence variations |
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| Disease | Lung adenocarcinoma (NCIt: C3512) | ||||||||||||||||||
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||||||||||||||||
| Hierarchy | Parent: CVCL_0023 (A-549) | ||||||||||||||||||
| Sex of cell | Male | ||||||||||||||||||
| Age at sampling | 58Y | ||||||||||||||||||
| Category | Cancer cell line | ||||||||||||||||||
| STR profile | Source(s): ATCC=CCL-185dCas9-KRAB Markers:
Run an STR similarity search on this cell line | ||||||||||||||||||
| Cross-references | |||||||||||||||||||
| Cell line collections (Providers) | ATCC; CCL-185dCas9-KRAB | ||||||||||||||||||
| Cell line databases/resources | cancercelllines; CVCL_VR73 | ||||||||||||||||||
| Encyclopedic resources | Wikidata; Q93311895 | ||||||||||||||||||
| Entry history | |||||||||||||||||||
| Entry creation | 25-Feb-2019 | ||||||||||||||||||
| Last entry update | 19-Dec-2024 | ||||||||||||||||||
| Version number | 11 | ||||||||||||||||||