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Cellosaurus MDA-MB-231 VIM RFP (CVCL_VR67)

[Text version]
Cell line name MDA-MB-231 VIM RFP
Accession CVCL_VR67
Resource Identification Initiative To cite this cell line use: MDA-MB-231 VIM RFP (RRID:CVCL_VR67)
Comments Group: Triple negative breast cancer (TNBC) cell line.
Population: Caucasian.
Characteristics: A red fluorescent protein (RFP) tag was added to the C-terminal of the VIM gene by CRISPR/Cas9. Allows to monitor the epithelial-to-mesenchymal transition (EMT) status of cells in vitro by monitoring RFP expression.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Gene deletion; HGNC; 1788; CDKN2B; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1097; BRAF; Simple; p.Gly464Val (c.1391G>T); ClinVar=VCV000040364; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg280Lys (c.839G>A); ClinVar=VCV000376657; Zygosity=Homozygous (from parent cell line).
Disease Breast adenocarcinoma (NCIt: C5214)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0062 (MDA-MB-231)
Sex of cell Female
Age at sampling 51Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-26MET

Markers:
AmelogeninX
CSF1PO12,13
D5S81812
D7S8208,9
D13S31713
D16S53912
TH017,9.3
TPOX8,9
vWA15,18

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Cross-references
Cell line collections (Providers) ATCC; HTB-26MET
Cell line databases/resources cancercelllines; CVCL_VR67
Encyclopedic resources Wikidata; Q95987359
Entry history
Entry creation25-Feb-2019
Last entry update10-Sep-2024
Version number13