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Cellosaurus A549 EML4-ALK-Luc2 (CVCL_VR66)

[Text version]
Cell line name A549 EML4-ALK-Luc2
Synonyms EML4-ALK Fusion-A549 Isogenic-Luc2
Accession CVCL_VR66
Resource Identification Initiative To cite this cell line use: A549 EML4-ALK-Luc2 (RRID:CVCL_VR66)
Comments Population: Caucasian.
Characteristics: Stably expresses firefly luciferase under the control of the human EF1a promoter.
Doubling time: ~23 hours (ATCC=CCL-185IG-LUC2).
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase (Note=With optimized codon usage for mammalian expression).
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_JK07 (A549 EML4-ALK)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): ATCC=CCL-185IG-LUC2

Markers:
AmelogeninX
CSF1PO10,12
D5S81811
D7S8208,11
D13S31711
D16S53911,12
TH018,9.3
TPOX8,11
vWA14

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Cross-references
Cell line collections (Providers) ATCC; CCL-185IG-LUC2
Cell line databases/resources cancercelllines; CVCL_VR66
Encyclopedic resources Wikidata; Q93311901
Entry history
Entry creation25-Feb-2019
Last entry update19-Dec-2024
Version number10