ID   Elf1-iCas9
AC   CVCL_VR49
DR   Wikidata; Q93539988
RX   PubMed=29466914;
CC   Characteristics: Cell line with robust Dox-inducible expression of Cas9. Can be used for rapid and highly efficient generation of biallelic knockout hPSCs for loss-of-function studies.
CC   Characteristics: Using TALEN two constructs SA-2A-PuroR-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele.
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C115 ! Elf1
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 13-11-18; Last updated: 10-09-24; Version: 8
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RX   PubMed=29466914; DOI=10.1080/15384101.2018.1442621; PMCID=PMC5969550;
RA   Ferreccio A., Mathieu J., Detraux D., Somasundaram L., Cavanaugh C.,
RA   Sopher B., Fischer K., Bello T., Hussein A.M., Levy S., Cook S.,
RA   Sidhu S.B., Artoni F., Palpant N.J., Reinecke H., Wang Y.-L.,
RA   Paddison P.J., Murry C.E., Jayadev S., Ware C.B., Ruohola-Baker H.;
RT   "Inducible CRISPR genome editing platform in naive human embryonic
RT   stem cells reveals JARID2 function in self-renewal.";
RL   Cell Cycle 17:535-549(2018).
//