Cellosaurus HEK293-SREBP1-T2A-luciferase-KI (CVCL_VR42)
| Cell line name | HEK293-SREBP1-T2A-luciferase-KI |
|---|---|
| Accession | CVCL_VR42 |
| Resource Identification Initiative | To cite this cell line use: HEK293-SREBP1-T2A-luciferase-KI (RRID:CVCL_VR42) |
| Comments | Characteristics: Useful for studying transcriptional regulation of the SREBP1 gene and for screening drugs or chemical molecules that regulate SREBP1 gene expression (PubMed=30232659). Characteristics: CRISPR/Cas9-mediated knock-in of a T2A-luciferase-CMV-eGFP-T2A-Neomycin construct at the C-terminal end of SREBP1. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0045 (HEK293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Publications | PubMed=30232659; DOI=10.1007/s10529-018-2608-2 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q94095267 |
| Entry history | |
| Entry creation | 13-Nov-2018 |
| Last entry update | 10-Sep-2024 |
| Version number | 6 |