Cellosaurus AR-EcoScreen GR KO M1 (CVCL_VN86)
| Cell line name | AR-EcoScreen GR KO M1 |
|---|---|
| Synonyms | AR-EcoScreen GR KnockOut Mutant 1 |
| Accession | CVCL_VN86 |
| Resource Identification Initiative | To cite this cell line use: AR-EcoScreen GR KO M1 (RRID:CVCL_VN86) |
| Comments | Characteristics: Used for rapid evaluation of in vitro androgen receptor transcriptional activation. The NR3C1/GR knockout renders the AR-EcoScreen assay insensitive to glucocorticoid activation. Characteristics: The luciferase reporter gene is under the control of a construct bearing four tandem repeats of a prostate C3 gene androgen-responsive element driven by a minimal heat shock protein promoter. Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:7978; NR3C1. Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:644; AR. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P27652; Renilla-type luciferase. Derived from site: In situ; Ovary; UBERON=UBERON_0000992. Cell type: Epithelial cell of ovary; CL=CL_2000064. |
| Species of origin | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029) |
| Hierarchy | Parent: CVCL_8544 (AR-EcoScreen) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=28803809; DOI=10.1016/j.tiv.2017.08.004 |
| Cross-references | |
| Cell line collections (Providers) | JCRB; JCRB1761 |
| Encyclopedic resources | Wikidata; Q93325715 |
| Entry history | |
| Entry creation | 07-Sep-2018 |
| Last entry update | 19-Dec-2024 |
| Version number | 8 |