Cellosaurus cell line CRISPRi Gen3C A4 (CVCL

Cross-references
Cell line name	CRISPRi Gen3C A4
Synonyms	WTC CRISPRi Gen3 clone A4; 3C-A4
Accession	CVCL_VM43
Resource Identification Initiative	To cite this cell line use: CRISPRi Gen3C A4 (RRID:CVCL_VM43)
Comments	Population: Japanese. Characteristics: Cell line with constitutive CRISPR interference (CRISPRi). Characteristics: Using TALEN a construct containing a CAG promoter and dCas9-KRAB is introduced in the AAVS1 safe harbor locus. Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB). Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511. Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_Y803 (GM25256)
Sex of cell	Male
Age at sampling	30Y
Category	Induced pluripotent stem cell
Publications	PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697 Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A., Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I., Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A., Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S., So P.-L., Conklin B.R. CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs. Cell Stem Cell 18:541-553(2016)
Encyclopedic resources	Wikidata; Q93458774
Entry history
Entry creation	07-Sep-2018
Last entry update	10-Sep-2024
Version number	9