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Cellosaurus CRISPRi Gen1C 5 (CVCL_VM38)

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Cell line name CRISPRi Gen1C 5
Synonyms WTC CRISPRi Gen1 clone 5; CRISPRi (Gen1C 5); 1C-5
Accession CVCL_VM38
Resource Identification Initiative To cite this cell line use: CRISPRi Gen1C 5 (RRID:CVCL_VM38)
Comments Population: Japanese.
Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 safe harbor locus.
Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Children:
CVCL_VQ27 (WTC CRISPRi HOPX g1)CVCL_VQ28 (WTC CRISPRi HOPX g4)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Publications

PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697
Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A., Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I., Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A., Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S., So P.-L., Conklin B.R.
CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs.
Cell Stem Cell 18:541-553(2016)

Cross-references
Encyclopedic resources Wikidata; Q93458763
Entry history
Entry creation07-Sep-2018
Last entry update10-Sep-2024
Version number10