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Cellosaurus CRISPRn Gen1C 22 (CVCL_VM34)

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Cell line name CRISPRn Gen1C 22
Synonyms WTC CRISPRn Gen1 clone 22; CRISPRi (Gen1C 22); 1C-n22
Accession CVCL_VM34
Resource Identification Initiative To cite this cell line use: CRISPRn Gen1C 22 (RRID:CVCL_VM34)
Comments Population: Japanese.
Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9 is introduced in the AAVS1 safe harbor locus.
Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Publications

PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697
Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A., Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I., Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A., Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S., So P.-L., Conklin B.R.
CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs.
Cell Stem Cell 18:541-553(2016)

Cross-references
Encyclopedic resources Wikidata; Q93458783
Entry history
Entry creation07-Sep-2018
Last entry update10-Sep-2024
Version number9