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Cellosaurus UCP1:mCherry H1 hES (CVCL_VJ25)

[Text version]
Cell line name UCP1:mCherry H1 hES
Accession CVCL_VJ25
Resource Identification Initiative To cite this cell line use: UCP1:mCherry H1 hES (RRID:CVCL_VJ25)
Comments Characteristics: Using CRISPR-Cas9 a IRES-PuroR-P2A-mCherry construct was integrated at the C-terminus of UCP1 (PubMed=29777802).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=29777802; DOI=10.1016/j.scr.2018.04.007; PMCID=PMC7376882
Mukherjee S., Zhang T., Lacko L.A., Tan L., Xiang J.Z.-Y., Butler J.M., Chen S.-B.
Derivation and characterization of a UCP1 reporter human ES cell line.
Stem Cell Res. 30:12-21(2018)

Cross-references
Encyclopedic resources Wikidata; Q98133852
Entry history
Entry creation07-Sep-2018
Last entry update10-Sep-2024
Version number8