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Cellosaurus WAe001-A-15 (CVCL_VF26)

[Text version]
Cell line name WAe001-A-15
Synonyms H1-MIR122-Tet-On; PB-TRE-MIR122; GIBHe007-A-15
Accession CVCL_VF26
Resource Identification Initiative To cite this cell line use: WAe001-A-15 (RRID:CVCL_VF26)
Comments From: Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences; Guangzhou; China.
Characteristics: Upon doxycycline-induction expresses miR-122. Developed to study the role of miR-122 in embryonic development, organogenesis, steatosis and tumorigenesis.
Characteristics: Transfected with a PB-TRE-miR-122-EF1a-rtTA-2A-puroR-2A-EGFP construct.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:31501; MIR122.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=29627726

Markers:
AmelogeninX,Y
CSF1PO12,13
D2S133818,19
D3S135815
D5S8189,11
D7S8208,12
D8S117912,13
D13S3178,11
D16S5399,13
D18S5117,18
D19S43313,15
D21S1128,32.2
FGA20,24
TH019.3
TPOX8,11
vWA15,17

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Publications

PubMed=29627726; DOI=10.1016/j.scr.2018.03.007
Liu Y.-L., Wu F.-M., Liu D., Wei R.-Z., Abbas N., Xia J.-H., Li D.-W., Wang H.-Y., Zhuang Y.-Q., Guo D.-S., Chen Y., Wu Y.-H., Ke X.-R., Tao J.-W., Yang F., Lai K.-Y., Shu X.-D., Li Y.-X.
Generation of WAe001-A-15, a human embryonic stem cell line with miR-122 doxycycline-inducible expression.
Stem Cell Res. 29:84-87(2018)

Cross-references
Cell line databases/resources hPSCreg; WAe001-A-15
Encyclopedic resources Wikidata; Q54993542
Entry history
Entry creation14-May-2018
Last entry update19-Dec-2024
Version number11