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Cellosaurus S2 EB1-DeltaPTIV-RacGAP50C-EGFP and TagRFP-alpha-tubulin (CVCL_UZ86)

[Text version]
Cell line name S2 EB1-DeltaPTIV-RacGAP50C-EGFP and TagRFP-alpha-tubulin
Accession CVCL_UZ86
Resource Identification Initiative To cite this cell line use: S2 EB1-DeltaPTIV-RacGAP50C-EGFP and TagRFP-alpha-tubulin (RRID:CVCL_UZ86)
Comments Group: Insect cell line.
Genetic integration: Method=Transfection; Gene=FlyBase_Gene; FBgn0027066; Eb1.
Genetic integration: Method=Transfection; Gene=FlyBase_Gene; FBgn0086356; tum (Note=With p.Pro533_Val536del).
Genetic integration: Method=Transfection; Gene=FlyBase_Gene; FBgn0003884; alphaTub84B.
Genetic integration: Method=Transfection; Gene=FPbase; S4HC8; TagRFP (Note=Red fluorescent protein derived from eqFP578).
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
Hierarchy Parent: CVCL_Z232 (Schneider 2)
Sex of cell Male
Age at sampling Late embryonic stage
Category Spontaneously immortalized cell line
Publications

PubMed=30758285; DOI=10.7554/eLife.38968; PMCID=PMC6398982
Verma V., Maresca T.J.
Microtubule plus-ends act as physical signaling hubs to activate RhoA during cytokinesis.
eLife 8:e38968.1-e38968.34(2019)

Cross-references
Encyclopedic resources Wikidata; Q98129255
Entry history
Entry creation25-Feb-2019
Last entry update19-Dec-2024
Version number9