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Cellosaurus HEK293 Cas9 (CVCL_UR28)

[Text version]
Cell line name HEK293 Cas9
Synonyms HEK-293 Cas9; 293 Cas9; 293[HEK-293] Cas9
Accession CVCL_UR28
Resource Identification Initiative To cite this cell line use: HEK293 Cas9 (RRID:CVCL_UR28)
Comments Characteristics: Using CRISPR/Cas9 a construct containing Cas9-2A-RFP-PA-Neo-PA was introduced in the AAVS1 safe harbor locus.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
STR profile Source(s): ATCC=CRL-1573Cas9

Markers:
AmelogeninX
CSF1PO12
D5S8188
D7S82011,12
D13S31712,14
D16S5399,13
TH017,9.3
TPOX11
vWA16,19

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Cross-references
Cell line collections (Providers) ATCC; CRL-1573Cas9
Encyclopedic resources Wikidata; Q94092805
Entry history
Entry creation25-Feb-2019
Last entry update10-Sep-2024
Version number8