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Cellosaurus 585B1-868 (CVCL_UP50)

[Text version]
Cell line name 585B1-868
Synonyms BTAG 585B1-868; 585B1 BTAG (XY)
Accession CVCL_UP50
Resource Identification Initiative To cite this cell line use: 585B1-868 (RRID:CVCL_UP50)
Comments From: Center for iPS Cell Research and Application, Kyoto University; Kyoto; Japan.
Population: Japanese.
Characteristics: Using TALEN a 2A-tdTomato construct was inserted at the C-terminal end of PRDM1 (BLIMP1) as well as 2A-EGFP construct at the C-terminal end of TFAP2C.
Genetic integration: Method=TALEN; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=TALEN; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_DQ07 (585B1)
Sex of cell Male
Age at sampling 30-39Y
Category Induced pluripotent stem cell
Publications

PubMed=26189426; DOI=10.1016/j.stem.2015.06.014
Sasaki K., Yokobayashi S., Nakamura T., Okamoto I., Yabuta Y., Kurimoto K., Ohta H., Moritoki Y., Iwatani C., Tsuchiya H., Nakamura S., Sekiguchi K., Sakuma T., Yamamoto T., Mori T., Woltjen K., Nakagawa M., Yamamoto T., Takahashi K., Yamanaka S., Saitou M.
Robust in vitro induction of human germ cell fate from pluripotent stem cells.
Cell Stem Cell 17:178-194(2015)

PubMed=28453617; DOI=10.1093/biolre/iox038
Yokobayashi S., Okita K., Nakagawa M., Nakamura T., Yabuta Y., Yamamoto T., Saitou M.
Clonal variation of human induced pluripotent stem cells for induction into the germ cell fate.
Biol. Reprod. 96:1154-1166(2017)

PubMed=30237246; DOI=10.1126/science.aat1674
Yamashiro C., Sasaki K., Yabuta Y., Kojima Y., Nakamura T., Okamoto I., Yokobayashi S., Murase Y., Ishikura Y., Shirane K., Sasaki H., Yamamoto T., Saitou M.
Generation of human oogonia from induced pluripotent stem cells in vitro.
Science 362:356-360(2018)

Cross-references
Encyclopedic resources Wikidata; Q93307890
Gene expression databases GEO; GSM2592205
GEO; GSM2592206
Entry history
Entry creation25-Feb-2019
Last entry update10-Sep-2024
Version number9