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Cellosaurus HK-2-KIM-1 (CVCL_UC05)

[Text version]
Cell line name HK-2-KIM-1
Accession CVCL_UC05
Resource Identification Initiative To cite this cell line use: HK-2-KIM-1 (RRID:CVCL_UC05)
Comments Population: Caucasian.
Characteristics: CRISPR/Cas9-mediated knock-in of eGFP-T2A-luciferase construct at the C-terminal end of HAVCR1/KIM-1.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P08659; Firefly luciferase.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03126; Human papillomavirus type 16 protein E6.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03129; Human papillomavirus type 16 protein E7.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
Derived from site: In situ; Kidney, proximal tubule; UBERON=UBERON_0004134.
Cell type: Epithelial cell of proximal tubule; CL=CL_0002306.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0302 (HK-2 [Human kidney])
Sex of cell Male
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=30260998; DOI=10.1371/journal.pone.0204487; PMCID=PMC6160069
Veach R.A., Wilson M.H.
CRISPR/Cas9 engineering of a KIM-1 reporter human proximal tubule cell line.
PLoS ONE 13:E0204487-E0204487(2018)

Cross-references
Encyclopedic resources Wikidata; Q94210556
Entry history
Entry creation13-Nov-2018
Last entry update10-Sep-2024
Version number9