ID   hCMEC/D3
AC   CVCL_U985
SY   HCMEC/D3; CMEC/D3; human Cortical Microvessels Endothelial Cells/D3
DR   BTO; BTO_0003339
DR   BCRJ; 0401
DR   CCRID; 5301HUM-KCB19022YJ
DR   ChEMBL-Cells; CHEMBL4513105
DR   CLS; 305024
DR   KCB; KCB 2019022YJ
DR   Millipore; SCC066
DR   PRIDE; PXD002292
DR   PubChem_Cell_line; CVCL_U985
DR   Ubigene; YC-C029
DR   Wikidata; Q54881892
RX   Patent=US8084254;
RX   PubMed=16141364;
RX   PubMed=23531482;
RX   PubMed=26718731;
RX   PubMed=30983357;
WW   Provider; Cedarlane; CLU512; https://www.cedarlanelabs.com/Products/Detail/CLU512?lob=AllProducts
WW   Provider; Cornell TTO; -; https://cornell.flintbox.com/technologies/bc9b2b68-4f6b-4071-bece-64241c21e438
CC   Group: Patented cell line.
CC   Registration: International Depositary Authority, Pasteur Institute Collection Nationale de Cultures de Micro-organismes (CNCM); I-3308.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Omics: Proteomics; Subcellular; Membrane; Quantitative.
CC   Omics: Proteomics; Subcellular; Secretome.
CC   Derived from site: In situ; Brain, temporal lobe, blood microvessel; UBERON=UBERON_0001871+UBERON_8410081.
CC   Cell type: Endothelial cell; CL=CL_0000115.
ST   Source(s): CCRID=5301HUM-KCB19022YJ; Millipore=SCC066; Technion Genomics Center
ST   Amelogenin: X
ST   CSF1PO: 10,12
ST   D10S1248: 14,15
ST   D12S391: 19.3,22
ST   D13S317: 11,14
ST   D16S539: 9,12
ST   D18S51: 12,19
ST   D1S1656: 15.3,17.3
ST   D21S11: 30,32.2
ST   D22S1045: 11,15
ST   D2S1338: 16,20
ST   D2S441: 13
ST   D3S1358: 16,17
ST   D5S818: 11
ST   D7S820: 10,12
ST   D8S1179: 15,16
ST   FGA: 22,26
ST   Penta D: 10,12
ST   Penta E: 5,10
ST   TH01: 7,9
ST   TPOX: 10,11
ST   vWA: 14,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 16-04-14; Last updated: 14-08-25; Version: 24
CH   CVCL_C8XS ! HCMEC/D3-Luc
CH   CVCL_E2Q2 ! HyCyte hCMEC/D3 hRNF213_p.R4810K_c.12429G>A
CH   CVCL_E1K0 ! HyCyte hCMEC/D3 KO-hRNF213
CH   CVCL_D8GX ! Ubigene hCMEC/D3 JAM2 KO
CH   CVCL_D8GY ! Ubigene hCMEC/D3 JAM3 KO
//
RX   PubMed=30983357; DOI=10.1021/acs.molpharmaceut.9b00114;
RA   Masuda, Takeshi
RA   Hoshiyama, Toshiki
RA   Uemura, Tatsuki
RA   Hirayama-Kurogi, Mio
RA   Ogata, Seiryo
RA   Furukawa, Arisu
RA   Couraud, Pierre-Olivier
RA   Furihata, Tomomi
RA   Ito, Shingo
RA   Ohtsuki, Sumio
RT   "Large-scale quantitative comparison of plasma transmembrane proteins
RT   between two human blood-brain barrier model cell lines, hCMEC/D3 and
RT   HBMEC/cibeta.";
RL   Mol. Pharm. 16:2162-2171(2019).
//
RX   PubMed=26718731; DOI=10.1016/j.jprot.2015.12.011;
RA   Llombart, Victor
RA   Garcia-Berrocoso, Teresa
RA   Bech-Serra, Joan Josep
RA   Simats, Alba
RA   Bustamante, Alejandro
RA   Giralt, Dolors
RA   Reverter-Branchat, Gemma
RA   Canals, Francesc
RA   Hernandez-Guillamon, Mar
RA   Montaner, Joan
RT   "Characterization of secretomes from a human blood brain barrier
RT   endothelial cells in-vitro model after ischemia by stable isotope
RT   labeling with aminoacids in cell culture (SILAC).";
RL   J. Proteomics 133:100-112(2016).
//
RX   PubMed=23531482; DOI=10.1186/2045-8118-10-16; PMCID=PMC3623852;
RA   Weksler, Babette B.
RA   Romero, Ignacio A.
RA   Couraud, Pierre-Olivier
RT   "The hCMEC/D3 cell line as a model of the human blood brain barrier.";
RL   Fluids Barriers CNS 10:16.1-16.10(2013).
//
RX   PubMed=16141364; DOI=10.1096/fj.04-3458fje;
RA   Weksler, Babette B.
RA   Subileau, Eva-Anne
RA   Perriere, Nicolas
RA   Charneau, Pierre
RA   Holloway, Karen A.
RA   Leveque, Mathilde
RA   Tricoire-Leignel, Helene
RA   Nicotra, Ambra
RA   Bourdoulous, Sandrine
RA   Turowski, Pawel
RA   Male, David K.
RA   Roux, Francoise
RA   Greenwood, John
RA   Romero, Ignacio A.
RA   Couraud, Pierre-Olivier
RT   "Blood-brain barrier-specific properties of a human adult brain
RT   endothelial cell line.";
RL   FASEB J. 19:1872-1874(2005).
//
RX   Patent=US8084254;
RA   Couraud, Pierre-Olivier
RA   Romero, Ignacio A.
RA   Weksler, Babette B.
RT   "Human blood brain barrier model.";
RL   Patent number US8084254, 27-Dec-2011.
//