ID   hCMEC/D3
AC   CVCL_U985
SY   HCMEC/D3; CMEC/D3; human Cortical Microvessels Endothelial Cells/D3
DR   BTO; BTO:0003339
DR   BCRJ; 0401
DR   CCRID; 5301HUM-KCB19022YJ
DR   ChEMBL-Cells; CHEMBL4513105
DR   KCB; KCB 2019022YJ
DR   Millipore; SCC066
DR   PRIDE; PXD002292
DR   PubChem_Cell_line; CVCL_U985
DR   Ubigene; YC-C029
DR   Wikidata; Q54881892
RX   Patent=US8084254;
RX   PubMed=16141364;
RX   PubMed=23531482;
RX   PubMed=26718731;
RX   PubMed=30983357;
WW   https://www.cedarlanelabs.com/Products/Detail/CLU512?lob=Cellutions
WW   https://cornell.flintbox.com/technologies/bc9b2b68-4f6b-4071-bece-64241c21e438
CC   Group: Patented cell line.
CC   Registration: International Depositary Authority, Pasteur Institute Collection Nationale de Cultures de Micro-organismes (CNCM); I-3308.
CC   Transfected with: HGNC; 11730; TERT.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Omics: Deep quantitative membrane proteome analysis.
CC   Omics: Secretome proteome analysis.
CC   Derived from site: In situ; Brain, temporal lobe, blood microvessel; UBERON=UBERON_0001871+UBERON_8410081.
CC   Cell type: Endothelial cell; CL=CL_0000115.
ST   Source(s): CCRID; Genomics_Center_BCF_Technion; Millipore
ST   Amelogenin: X
ST   CSF1PO: 10,12
ST   D10S1248: 14,15
ST   D12S391: 19.3,22
ST   D13S317: 11,14
ST   D16S539: 9,12
ST   D18S51: 12,19
ST   D1S1656: 15.3,17.3
ST   D21S11: 30,32.2
ST   D22S1045: 11,15
ST   D2S1338: 16,20
ST   D2S441: 13
ST   D3S1358: 16,17
ST   D5S818: 11
ST   D7S820: 10,12
ST   D8S1179: 15,16
ST   FGA: 22,26
ST   Penta D: 10,12
ST   Penta E: 5,10
ST   TH01: 7,9
ST   TPOX: 10,11
ST   vWA: 14,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 16-04-14; Last updated: 05-10-23; Version: 19
//
RX   Patent=US8084254;
RA   Couraud P.-O., Romero I.A., Weksler B.B.;
RT   "Human blood brain barrier model.";
RL   Patent number US8084254, 27-Dec-2011.
//
RX   PubMed=16141364; DOI=10.1096/fj.04-3458fje;
RA   Weksler B.B., Subileau E.-A., Perriere N., Charneau P., Holloway K.,
RA   Leveque M., Tricoire-Leignel H., Nicotra A., Bourdoulous S.,
RA   Turowski P., Male D.K., Roux F., Greenwood J., Romero I.A.,
RA   Couraud P.-O.;
RT   "Blood-brain barrier-specific properties of a human adult brain
RT   endothelial cell line.";
RL   FASEB J. 19:1872-1874(2005).
//
RX   PubMed=23531482; DOI=10.1186/2045-8118-10-16;
RA   Weksler B.B., Romero I.A., Couraud P.-O.;
RT   "The hCMEC/D3 cell line as a model of the human blood brain barrier.";
RL   Fluids Barriers CNS 10:16.1-16.10(2013).
//
RX   PubMed=26718731; DOI=10.1016/j.jprot.2015.12.011;
RA   Llombart V., Garcia-Berrocoso T., Bech-Serra J.J., Simats A.,
RA   Bustamante A., Giralt D., Reverter-Branchat G., Canals F.,
RA   Hernandez-Guillamon M., Montaner J.;
RT   "Characterization of secretomes from a human blood brain barrier
RT   endothelial cells in-vitro model after ischemia by stable isotope
RT   labeling with aminoacids in cell culture (SILAC).";
RL   J. Proteomics 133:100-112(2016).
//
RX   PubMed=30983357; DOI=10.1021/acs.molpharmaceut.9b00114;
RA   Masuda T., Hoshiyama T., Uemura T., Hirayama-Kurogi M., Ogata S.,
RA   Furukawa A., Couraud P.-O., Furihata T., Ito S., Ohtsuki S.;
RT   "Large-scale quantitative comparison of plasma transmembrane proteins
RT   between two human blood-brain barrier model cell lines, hCMEC/D3 and
RT   HBMEC/cibeta.";
RL   Mol. Pharm. 16:2162-2171(2019).
//