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Cellosaurus tsBN2 (CVCL_U467)

[Text version]
Cell line name tsBN2
Accession CVCL_U467
Resource Identification Initiative To cite this cell line use: tsBN2 (RRID:CVCL_U467)
Comments Characteristics: At the nonpermissive temperature (39.5 Celsius), the chromatin of tsBN2 cells is prematurely condensed, and the cells die (PubMed=694723).
Transformant: ChEBI; CHEBI_21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Sequence variations
  • Mutation; UniProtKB; P23800; Rcc1; Simple; p.Ser256Phe (c.767C>T); Zygosity=Homozygous (PubMed=2300055).
Species of origin Mesocricetus auratus (Golden hamster) (NCBI Taxonomy: 10036)
Hierarchy Parent: CVCL_1915 (BHK-21 clone 13)
Children:
CVCL_E3K4 (tsBN2-N9)
Sex of cell Male
Age at sampling 1D
Category Spontaneously immortalized cell line
Publications

PubMed=694723; DOI=10.1007/BF01542846
Nishimoto T., Basilico C.
Analysis of a method for selecting temperature-sensitive mutants of BHK cells.
Somatic Cell Genet. 4:323-340(1978)

PubMed=3785187; DOI=10.1128/mcb.6.6.2027-2032.1986; PMCID=PMC367742
Kai R., Ohtsubo M., Sekiguchi M., Nishimoto T.
Molecular cloning of a human gene that regulates chromosome condensation and is essential for cell proliferation.
Mol. Cell. Biol. 6:2027-2032(1986)

PubMed=2300055; DOI=10.1128/mcb.10.2.577-584.1990; PMCID=PMC360843
Uchida S., Sekiguchi T., Nishitani H., Miyauchi K., Ohtsubo M., Nishimoto T.
Premature chromosome condensation is induced by a point mutation in the hamster RCC1 gene.
Mol. Cell. Biol. 10:577-584(1990)

PubMed=1851087; DOI=10.1002/j.1460-2075.1991.tb07675.x; PMCID=PMC452820
Nishitani H., Ohtsubo M., Yamashita K., Iida H., Pines J., Yasudo H., Shibata Y., Hunter T., Nishimoto T.
Loss of RCC1, a nuclear DNA-binding protein, uncouples the completion of DNA replication from the activation of cdc2 protein kinase and mitosis.
EMBO J. 10:1555-1564(1991)

Cross-references
Cell line collections (Providers) RCB; RCB1264
Cell line databases/resources CLO; CLO_0051408
Encyclopedic resources Wikidata; Q54973104
Entry history
Entry creation03-Feb-2014
Last entry update19-Dec-2024
Version number9