Cellosaurus cell line MAC-T (CVCL_U226)

Cellosaurus MAC-T (CVCL_U226)

Cell line name

MAC-T

Synonyms

Mac-T; MacT; MAC-T3; Mammary Alveolar Cells-large T antigen

Accession

CVCL_U226

Resource Identification Initiative

To cite this cell line use: MAC-T (RRID:CVCL_U226)

Comments

Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10274.
Doubling time: ~17 hours (PubMed=1659986); 48 hours (Note=In serum-free medium) (PubMed=7540186).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
Omics: Transcriptomics; Microarray.
Anecdotal: Nexia, a former Canadian biotech company was orignally developed to make lactose-free free milk using MAC-T.
Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
Cell type: Mammary alveolar epithelial cell; CL=CL_0002325.

Species of origin

Bos taurus (Bovine) (NCBI Taxonomy: 9913)
Breed/subspecies: Holstein; VBO=VBO_0000237.

Hierarchy

Parent: CVCL_B3UP (LMH17)
Children:

CVCL_E1LV (HyCyte MAC-T KO-cwHMOX1)	CVCL_E1LW (HyCyte MAC-T KO-cwNFE2L2)	CVCL_D6C0 (HyCyte MAC-T KO-cwNUP62)
CVCL_E1LX (HyCyte MAC-T KO-cwSIRT5)	CVCL_VN97 (MAC-T BLV)	CVCL_U230 (MAC-T CU-1)
CVCL_U231 (MAC-T CU-2)	CVCL_U232 (MAC-T CU-3)	CVCL_E8BZ (MAC-T spCas9 #1)
CVCL_E8C0 (MAC-T spCas9 #2)	CVCL_E8C1 (MAC-T spCas9 #3)	CVCL_E8C2 (MAC-T spCas9 #4)
CVCL_HG41 (MD-IGF-I)

Sex of cell

Female

Age at sampling

Adult

Category

Transformed cell line

Publications

CLPUB00665
Huynh H.T.
Establishment of bovine mammary epithelial cell lines: an in vitro model for lactation.
Thesis MSc (1990); McGill University Montreal; Montreal; Canada

PubMed=1659986; DOI=10.1016/0014-4827(91)90422-Q
Huynh H.T., Robitaille G., Turner J.D.
Establishment of bovine mammary epithelial cells (MAC-T): an in vitro model for bovine lactation.
Exp. Cell Res. 197:191-199(1991)

PubMed=1423654; DOI=10.1016/S0309-1651(06)80052-4
Rejman J.J., Oliver S.P., Muenchen R.A., Turner J.D.
Proliferation of the MAC-T bovine mammary epithelial cell line in the presence of mammary secretion whey proteins.
Cell Biol. Int. Rep. 16:993-1001(1992)

Patent=US5227301
Turner J.D., Huynh H.T.
Immortalized bovine mammary epithelial cell line.
Patent number US5227301, 13-Jul-1993

Patent=US5455164
Turner J.D.
Ruminant immortalized mammary epithelial cell lines.
Patent number US5455164, 03-Oct-1995

PubMed=7540186; DOI=10.3168/jds.S0022-0302(95)76662-0
Zavizion B., Gorewit R.C., Politis I.
Subcloning the MAC-T bovine mammary epithelial cell line: morphology, growth properties, and cytogenetic analysis of clonal cells.
J. Dairy Sci. 78:515-527(1995)

PubMed=14550508; DOI=10.1016/S0739-7240(03)00062-6
Berry S.D.K., Weber Nielsen M.S., Sejrsen K., Pearson R.E., Boyle P.L., Akers R.M.
Use of an immortalized bovine mammary epithelial cell line (MAC-T) to measure the mitogenic activity of extracts from heifer mammary tissue: effects of nutrition and ovariectomy.
Domest. Anim. Endocrinol. 25:245-253(2003)

DOI=10.4172/2329-888X.1000103
Hosseini A., Sharma R., Bionaz M., Loor J.J.
Transcriptomics comparisons of Mac-T cells versus mammary tissue during late pregnancy and peak lactation.
Adv. Dairy Res. 1:103-103(2013)

PubMed=26659725; DOI=10.1152/ajpcell.00261.2015
Arevalo Turrubiarte M., Perruchot M.-H., Finot L., Mayeur F., Dessauge F.
Phenotypic and functional characterization of two bovine mammary epithelial cell lines in 2D and 3D models.
Am. J. Physiol. Cell Physiol. 310:C348-C356(2016)

PubMed=30055215; DOI=10.1016/j.virusres.2018.07.013
Martinez Cuesta L., Nieto Farias M.V., Lendez P.A., Barone L., Perez S.E., Dolcini G.L., Ceriani M.C.
Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV).
Virus Res. 256:11-16(2018)

Cross-references

Cell line collections (Providers)

BCRJ; 0315

Encyclopedic resources

Wikidata; Q54903957

Gene expression databases

Entry history

Entry creation

03-Feb-2014

Last entry update

10-Apr-2025

Version number

18