Cellosaurus logo
expasy logo

Cellosaurus NMuMG/Fucci2 (CVCL_U135)

[Text version]
Cell line name NMuMG/Fucci2
Synonyms NMuMG/Fucci2 #5; NMuMG-Fucci2
Accession CVCL_U135
Resource Identification Initiative To cite this cell line use: NMuMG/Fucci2 (RRID:CVCL_U135)
Comments Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci2 (Fluorescent Ubiquitination-based Cell Cycle Indicator 2) construct.
Characteristics: Transfected with the Fucci2 probe which consist of the fusion of mCherry) to the ubiquitination domain of human CDT1 (AA 30-120) and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (AA 1-110).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Met1_Thr29del and p.Ser121_Leu546del).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del).
Genetic integration: Method=Transfection; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=Transfection; Gene=FPbase; YUJWJ; Venus (Note=Bright yellow fluorescent protein derived from GFP).
Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: Namru.
Hierarchy Parent: CVCL_0075 (NMuMG)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=21226962; DOI=10.1186/1471-2121-12-2; PMCID=PMC3277280
Sakaue-Sawano A., Kobayashi T., Ohtawa K., Miyawaki A.
Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.
BMC Cell Biol. 12:2.1-2.12(2011)

PubMed=24786503; DOI=10.1101/pdb.prot080408
Sakaue-Sawano A., Miyawaki A.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progressions with Fucci technology.
Cold Spring Harb. Protoc. 2014:Pdb.prot080408-Pdb.prot080408(2014)

Cross-references
Cell line collections (Providers) RCB; RCB2868
Cell line databases/resources CLO; CLO_0050968
Encyclopedic resources Wikidata; Q54930851
Entry history
Entry creation03-Feb-2014
Last entry update19-Dec-2024
Version number12