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Cellosaurus K-562 CRISPRa+SaCas9 clone C7 (CVCL_RY27)

[Text version]
Cell line name K-562 CRISPRa+SaCas9 clone C7
Accession CVCL_RY27
Resource Identification Initiative To cite this cell line use: K-562 CRISPRa+SaCas9 clone C7 (RRID:CVCL_RY27)
Comments Population: Caucasian.
Characteristics: This cell line is transfected by a nuclease-deficient mutant of SpCas9 (dCas9) under the control of a promoter containing 4 copies of the activating domain of HSV-1 VP16 (termed VP64) and to 24 copies of a short peptide epitope (from yeast GCN4) which is termed SunTag.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; J7RUA5; Staphylococcus aureus Cas9.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; 76; ABL1 + HGNC; 1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).
Disease Blast phase chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C9110)
Chronic myeloid leukemia (ORDO: Orphanet_521)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_RY26 (K-562 CRISPRa)
Sex of cell Female
Age at sampling 53Y
Category Cancer cell line
Publications

PubMed=29334369; DOI=10.1038/nbt.4062; PMCID=PMC6072461
Boettcher M., Tian R.-L., Blau J.A., Markegard E., Wagner R.T., Wu D., Mo X.-L., Biton A., Zaitlen N., Fu H.-A., McCormick F., Kampmann M., McManus M.T.
Dual gene activation and knockout screen reveals directional dependencies in genetic networks.
Nat. Biotechnol. 36:170-178(2018)

Cross-references
Encyclopedic resources Wikidata; Q54899216
Entry history
Entry creation14-May-2018
Last entry update10-Sep-2024
Version number13