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Cellosaurus HEK293 pcDNA5/FRT/TO-KAP1 (CVCL_RX69)

[Text version]
Cell line name HEK293 pcDNA5/FRT/TO-KAP1
Synonyms pcDNA5/FRT/TO-KAP1-HEK293
Accession CVCL_RX69
Resource Identification Initiative To cite this cell line use: HEK293 pcDNA5/FRT/TO-KAP1 (RRID:CVCL_RX69)
Comments Characteristics: Can express TRIM28 inducibly by the addition of doxycyclin (DOX).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 16384; TRIM28.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_U427 (Flp-In-T-REx-293)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=26081272; DOI=10.1556/018.66.2015.2.3
Liu X.-Y., Khan M.A., Cheng J.-L., Wei C.-L., Zhang L.-M., Fu J.-J.
Establishment of stable cell line for inducing KAP1 protein expression.
Acta Biol. Hung. 66:161-168(2015)

Cross-references
Encyclopedic resources Wikidata; Q54882436
Entry history
Entry creation05-Mar-2018
Last entry update10-Sep-2024
Version number9