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Cellosaurus BWZ.36/CD8alpha (CVCL_RW75)

[Text version]
Cell line name BWZ.36/CD8alpha
Synonyms BWZ.36 CD8 alpha; BWZ.36.CD8 alpha
Accession CVCL_RW75
Resource Identification Initiative To cite this cell line use: BWZ.36/CD8alpha (RRID:CVCL_RW75)
Comments Group: Hybridoma fusion partner cell line.
Characteristics: Transfected with the NFAT-lacZ construct containing the lacZ reporter gene under control of the NFAT response element.
Characteristics: Does not express functional TCR alpha and beta chains (from parent cell line).
Selected for resistance to: ChEBI; CHEBI_472805; Ouabain.
Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
Genetic integration: Method=Transduction; Gene=MGI; MGI:88346; Cd8a.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transformant: NCIt; C44386; Gamma radiation.
Derived from site: In situ; Thymus; UBERON=UBERON_0002370.
Disease Mouse lymphoma (NCIt: C21602)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: AKR/J.
Hierarchy Parent: CVCL_RW63 (BWZ.36)
Category Cancer cell line
Publications

PubMed=8186188; DOI=10.1093/intimm/6.3.369
Sanderson S., Shastri N.
LacZ inducible, antigen/MHC-specific T cell hybrids.
Int. Immunol. 6:369-376(1994)

Cross-references
Anatomy/cell type resources BTO; BTO_0006295
Encyclopedic resources Wikidata; Q54798743
Entry history
Entry creation05-Mar-2018
Last entry update19-Dec-2024
Version number10