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Cellosaurus CHO DRA10 (CVCL_RW58)

[Text version]
Cell line name CHO DRA10
Synonyms CHO-K1 DRA10; DRA10
Accession CVCL_RW58
Resource Identification Initiative To cite this cell line use: CHO DRA10 (RRID:CVCL_RW58)
Comments Characteristics: Transfected with the DRneo construct which contains a hygromycin resistance gene and two non-functional copies of the neomycin resistance gene. This cell line is sensitive to G418 but recombination recreates a functional Neo gene and recombinants become G418 resistant.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=8799130; DOI=10.1073/pnas.93.17.8929; PMCID=PMC38571
Liang F., Romanienko P.J., Weaver D.T., Jeggo P.A., Jasin M.
Chromosomal double-strand break repair in Ku80-deficient cells.
Proc. Natl. Acad. Sci. U.S.A. 93:8929-8933(1996)

Cross-references
Encyclopedic resources Wikidata; Q54812582
Entry history
Entry creation05-Mar-2018
Last entry update10-Sep-2024
Version number10