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Cellosaurus HA-R-Spondin1-Fc 293T (CVCL_RU08)

[Text version]
Cell line name HA-R-Spondin1-Fc 293T
Synonyms HA-R-Spondin-Fc293T
Accession CVCL_RU08
Resource Identification Initiative To cite this cell line use: HA-R-Spondin1-Fc 293T (RRID:CVCL_RU08)
Comments Characteristics: Expresses mouse Fc-Rspo1 which consist of the fusion of mouse IgG2a Fc region to the N-terminal of Rspo1 with a N-terminal HA epitope tag (AMSBIO).
Genetic integration: Method=Transfection; Gene=MGI; MGI:2183426; Rspo1 (Note=With a N-terminal HA tag).
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Caution: Could be identical to 293T R-Spondin1 expressing (Cellosaurus=CVCL_RU09) distributed by Millipore. Both cell line product pages cite PubMed=19398967 but the Millipore page states "Cell line is not the published cell line from the Kuo lab, but rather a related cell line developed in the same lab using the reference protocol".
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Web pages Provider; AMSBIO; -; https://www.amsbio.com/r-spondin-rspo1-cells/
Publications

PubMed=19398967; DOI=10.1038/nm.1951; PMCID=PMC2919216
Ootani A., Li X.-N., Sangiorgi E., Ho Q.T., Ueno H., Toda S., Sugihara H., Fujimoto K., Weissman I.L., Capecchi M.R., Kuo C.J.
Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche.
Nat. Med. 15:701-706(2009)

Cross-references
Encyclopedic resources Wikidata; Q54872280
Entry history
Entry creation05-Mar-2018
Last entry update10-Apr-2025
Version number10