Cellosaurus HCT 116 CMV-OsTIR1 (CVCL_RJ11)
Cell line name | HCT 116 CMV-OsTIR1 |
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Synonyms | HCT116 CMV-OsTIR1 |
Accession | CVCL_RJ11 |
Resource Identification Initiative | To cite this cell line use: HCT 116 CMV-OsTIR1 (RRID:CVCL_RJ11) |
Comments | Population: Caucasian. Characteristics: Parent cell line to study the rapid depletion of a protein. The expressed rice TIR1 can form a functional SCF(OsTIR1) E3 ligase complex with the endogenous components in human cells. In the presence of auxin, a protein of interest fused with mAID is rapidly degraded upon poly-ubiquitylation. Characteristics: Using CRISPR/Cas9 a CMV-OsTIR1-PuroR (pMK232) vector was introduced in both alleles of the AAVS1 safe harbor locus. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q7XVM8; Oryza sativa subsp. japonica TIR1-like protein Os04g0395600. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
Sequence variations |
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Disease | Colon carcinoma (NCIt: C4910) |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_0291 (HCT 116) |
Sex of cell | Male |
Age at sampling | 48Y |
Category | Cancer cell line |
Publications | PubMed=27052166; DOI=10.1016/j.celrep.2016.03.001 |
Cross-references | |
Cell line collections (Providers) | RCB; RCB4662 |
Cell line databases/resources | cancercelllines; CVCL_RJ11 |
Encyclopedic resources | Wikidata; Q54881927 |
Entry history | |
Entry creation | 05-Mar-2018 |
Last entry update | 19-Dec-2024 |
Version number | 13 |