Cellosaurus HCT116-hNIS-Neo/Fluc-Puro (CVCL_RA43)
Cell line name | HCT116-hNIS-Neo/Fluc-Puro |
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Accession | CVCL_RA43 |
Resource Identification Initiative | To cite this cell line use: HCT116-hNIS-Neo/Fluc-Puro (RRID:CVCL_RA43) |
Comments | Population: Caucasian. Characteristics: Reporter cell line, transduced with both the LV-hNIS-IRES-Neo and LV-Fluc-P2A-Puro vectors. LV-hNIS-IRES-Neo encodes for human SLC5A5 linked via an internal ribosomal entry site (IRES) to the Neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-Fluc-P2A-Puro encodes for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL009). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11040; SLC5A5. Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
Sequence variations |
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Disease | Colon carcinoma (NCIt: C4910) |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_0291 (HCT 116) |
Sex of cell | Male |
Age at sampling | 48Y |
Category | Cancer cell line |
Cross-references | |
Cell line collections (Providers) | Imanis; CL009 |
Cell line databases/resources | cancercelllines; CVCL_RA43 |
Encyclopedic resources | Wikidata; Q54882041 |
Entry history | |
Entry creation | 05-Mar-2018 |
Last entry update | 19-Dec-2024 |
Version number | 14 |