Cellosaurus LL/2-Fluc-Neo/eGFP-Puro (CVCL_RA23)
| Cell line name | LL/2-Fluc-Neo/eGFP-Puro |
|---|---|
| Accession | CVCL_RA23 |
| Resource Identification Initiative | To cite this cell line use: LL/2-Fluc-Neo/eGFP-Puro (RRID:CVCL_RA23) |
| Comments | Characteristics: Reporter cell line, transfected with both the LV-Fluc-P2A-Neo and LV-eGFP-PGKp-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGKp-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the PuroR gene under the control of the mouse PGK promoter (Imanis=CL073). Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Lung; UBERON=UBERON_0002048. |
| Disease | Malignant tumors of the mouse pulmonary system (NCIt: C21769) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6. |
| Hierarchy | Parent: CVCL_4358 (LL/2 (LLC1)) |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Imanis; CL073 |
| Encyclopedic resources | Wikidata; Q54902681 |
| Entry history | |
| Entry creation | 05-Mar-2018 |
| Last entry update | 10-Sep-2024 |
| Version number | 9 |