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Cellosaurus LL/2-Fluc-Neo/eGFP-Puro (CVCL_RA23)

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Cell line name LL/2-Fluc-Neo/eGFP-Puro
Accession CVCL_RA23
Resource Identification Initiative To cite this cell line use: LL/2-Fluc-Neo/eGFP-Puro (RRID:CVCL_RA23)
Comments Characteristics: Reporter cell line, transduced with both the LV-Fluc-P2A-Neo and LV-eGFP-PGKp-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGKp-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the PuroR gene under the control of the mouse PGK promoter (Imanis=CL073).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Disease Malignant tumors of the mouse pulmonary system (NCIt: C21769)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Parent: CVCL_4358 (LL/2 (LLC1))
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL073
Encyclopedic resources Wikidata; Q54902681
Entry history
Entry creation05-Mar-2018
Last entry update19-Dec-2024
Version number10