Cellosaurus Hepa1-6-Fluc-Neo/eGFP-Puro (CVCL_RA20)
| Cell line name | Hepa1-6-Fluc-Neo/eGFP-Puro |
|---|---|
| Accession | CVCL_RA20 |
| Resource Identification Initiative | To cite this cell line use: Hepa1-6-Fluc-Neo/eGFP-Puro (RRID:CVCL_RA20) |
| Comments | Characteristics: Reporter cell line, transfected with both the LV-Fluc-P2A-Neo and LV-eGFP-PGKp-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGKp-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the PuroR gene under the control of the mouse PGK promoter (Imanis=CL133). Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Liver; UBERON=UBERON_0002107. |
| Disease | Hepatocellular carcinoma of the mouse (NCIt: C38756) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: C57L. |
| Hierarchy | Parent: CVCL_0327 (Hepa 1-6) |
| Sex of cell | Female |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Imanis; CL133 |
| Encyclopedic resources | Wikidata; Q54882824 |
| Entry history | |
| Entry creation | 05-Mar-2018 |
| Last entry update | 10-Sep-2024 |
| Version number | 9 |