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Cellosaurus C1498-Fluc-Neo/eGFP-Puro (CVCL_QZ97)

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Cell line name C1498-Fluc-Neo/eGFP-Puro
Accession CVCL_QZ97
Resource Identification Initiative To cite this cell line use: C1498-Fluc-Neo/eGFP-Puro (RRID:CVCL_QZ97)
Comments Characteristics: Reporter cell line, transduced with both the LV-Fluc-P2A-Neo and LV-eGFP-PGKp-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGKp-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the PuroR gene under the control of the mouse PGK promoter (Imanis=CL145).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Cell type: Lymphoblast; CL=CL_0017005.
Disease Mouse leukemia (NCIt: C21604)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6J.
Hierarchy Parent: CVCL_3494 (C1498)
Sex of cell Female
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL145
Encyclopedic resources Wikidata; Q54807860
Entry history
Entry creation05-Mar-2018
Last entry update19-Dec-2024
Version number8