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Cellosaurus A549-iRFP-Puro (CVCL_QZ85)

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Cell line name A549-iRFP-Puro
Accession CVCL_QZ85
Resource Identification Initiative To cite this cell line use: A549-iRFP-Puro (RRID:CVCL_QZ85)
Comments Population: Caucasian.
Characteristics: Reporter cell line, transduced with the LV-iRFP-P2A-Puro vector encoding for iRFP linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL082).
Genetic integration: Method=Transduction; Gene=FPbase; DGXFA; iRFP (Note=iRFP713, near infra-red fluorescent protein from R.palustris).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0023 (A-549)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL082
Cell line databases/resources cancercelllines; CVCL_QZ85
Encyclopedic resources Wikidata; Q54607035
Entry history
Entry creation05-Mar-2018
Last entry update19-Dec-2024
Version number12