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Cellosaurus A549-hNIS-Neo/Fluc-Puro (CVCL_QZ82)

[Text version]
Cell line name A549-hNIS-Neo/Fluc-Puro
Accession CVCL_QZ82
Resource Identification Initiative To cite this cell line use: A549-hNIS-Neo/Fluc-Puro (RRID:CVCL_QZ82)
Comments Population: Caucasian.
Characteristics: Reporter cell line, transduced with both the LV-hNIS-IRES-Neo and LV-Fluc-P2A-Puro vectors. LV-hNIS-IRES-Neo encodes for human SLC5A5 linked via an internal ribosomal entry site (IRES) to the Neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-Fluc-P2A-Puro encodes for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL083).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11040; SLC5A5.
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0023 (A-549)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Publications

PubMed=30674994; DOI=10.1038/s41417-019-0081-2; PMCID=PMC7170803
Vandergaast R., Khongwichit S., Jiang H.-L., DeGrado T.R., Peng K.-W., Smith D.R., Russell S.J., Suksanpaisan L.
Enhanced noninvasive imaging of oncology models using the NIS reporter gene and bioluminescence imaging.
Cancer Gene Ther. 27:179-188(2020)

Cross-references
Cell line collections (Providers) Imanis; CL083
Cell line databases/resources cancercelllines; CVCL_QZ82
Encyclopedic resources Wikidata; Q54607028
Entry history
Entry creation05-Mar-2018
Last entry update19-Dec-2024
Version number13