Cellosaurus A549-Fluc-Neo/eGFP-Puro (CVCL_QZ77)
| Cell line name | A549-Fluc-Neo/eGFP-Puro |
|---|---|
| Accession | CVCL_QZ77 |
| Resource Identification Initiative | To cite this cell line use: A549-Fluc-Neo/eGFP-Puro (RRID:CVCL_QZ77) |
| Comments | Population: Caucasian. Characteristics: Reporter cell line, transduced with both the LV-Fluc-P2A-Neo and LV-eGFP-PGKp-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGKp-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the PuroR gene under the control of the mouse PGK promoter (Imanis=CL097). Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Lung; UBERON=UBERON_0002048. |
| Sequence variations |
|
| Disease | Lung adenocarcinoma (NCIt: C3512) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0023 (A-549) |
| Sex of cell | Male |
| Age at sampling | 58Y |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Imanis; CL097 |
| Cell line databases/resources | cancercelllines; CVCL_QZ77 |
| Encyclopedic resources | Wikidata; Q54607020 |
| Entry history | |
| Entry creation | 05-Mar-2018 |
| Last entry update | 19-Dec-2024 |
| Version number | 13 |