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Cellosaurus A549-Fluc-Neo/eGFP-Puro (CVCL_QZ77)

[Text version]
Cell line name A549-Fluc-Neo/eGFP-Puro
Accession CVCL_QZ77
Resource Identification Initiative To cite this cell line use: A549-Fluc-Neo/eGFP-Puro (RRID:CVCL_QZ77)
Comments Population: Caucasian.
Characteristics: Reporter cell line, transfected with both the LV-Fluc-P2A-Neo and LV-eGFP-PGK-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGK-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the pac gene under the control of the mouse PGK promoter.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P08659; Firefly luciferase.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0023 (A-549)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL097
Cell line databases/resources cancercelllines; CVCL_QZ77
Encyclopedic resources Wikidata; Q54607020
Entry history
Entry creation05-Mar-2018
Last entry update05-Oct-2023
Version number10