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Cellosaurus A375-iRFP-Neo/hNIS-Puro (CVCL_QZ75)

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Cell line name A375-iRFP-Neo/hNIS-Puro
Accession CVCL_QZ75
Resource Identification Initiative To cite this cell line use: A375-iRFP-Neo/hNIS-Puro (RRID:CVCL_QZ75)
Comments Population: Caucasian.
Characteristics: Reporter cell line, transfected with both the LV-iRFP-P2A-Neo and LV-hNIS-P2A-Puro vectors. LV-iRFP-P2A-Neo encodes for iRFP linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-hNIS-P2A-Puro encodes for human SLC5A5 linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL090).
Genetic integration: Method=Transfection; Gene=HGNC; 11040; SLC5A5.
Genetic integration: Method=Transfection; Gene=FPbase; DGXFA; iRFP (Note=iRFP713, near infra-red fluorescent protein from R.palustris).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu61Ter (c.181G>T) (p.Gly75Val, c.224G>T); ClinVar=VCV000376309; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu69Ter (c.205G>T) (p.Gly83Val, c.248G>T); ClinVar=VCV000376308; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0132 (A-375)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL090
Cell line databases/resources cancercelllines; CVCL_QZ75
Encyclopedic resources Wikidata; Q54606797
Entry history
Entry creation05-Mar-2018
Last entry update10-Sep-2024
Version number14