Cellosaurus A375-iRFP-Neo/Fluc-Puro (CVCL_QZ74)
| Cell line name | A375-iRFP-Neo/Fluc-Puro |
|---|---|
| Accession | CVCL_QZ74 |
| Resource Identification Initiative | To cite this cell line use: A375-iRFP-Neo/Fluc-Puro (RRID:CVCL_QZ74) |
| Comments | Population: Caucasian. Characteristics: Reporter cell line, transfected with both the LV-iRFP-P2A-Neo and LV-Fluc-P2A-Puro vectors. LV-iRFP-P2A-Neo encodes for iRFP linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-Fluc-P2A-Puro encodes for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL089). Genetic integration: Method=Transfection; Gene=FPbase; DGXFA; iRFP (Note=iRFP713, near infra-red fluorescent protein from R.palustris). Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511. |
| Sequence variations |
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| Disease | Amelanotic melanoma (NCIt: C3802) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0132 (A-375) |
| Sex of cell | Female |
| Age at sampling | 54Y |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Imanis; CL089 |
| Cell line databases/resources | cancercelllines; CVCL_QZ74 |
| Encyclopedic resources | Wikidata; Q54606795 |
| Entry history | |
| Entry creation | 05-Mar-2018 |
| Last entry update | 10-Sep-2024 |
| Version number | 14 |