Cellosaurus 4T1-mNIS-Puro/Fluc-Neo (CVCL_QZ65)
| Cell line name | 4T1-mNIS-Puro/Fluc-Neo |
|---|---|
| Synonyms | 4T1-Fluc-Neo/mNIS-Puro |
| Accession | CVCL_QZ65 |
| Resource Identification Initiative | To cite this cell line use: 4T1-mNIS-Puro/Fluc-Neo (RRID:CVCL_QZ65) |
| Comments | Characteristics: Reporter cell line, transfected with both the LV-mNIS-PGKp-PuroR and LV-Fluc-P2A-Neo vectors. LV-mNIS-PGKp-PuroR encodes for mouse Slc5a5 under control of the spleen focus-forming virus (SFFV) promoter and the PuroR gene under the control of the mouse PGK promoter. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL066). Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG). Genetic integration: Method=Transfection; Gene=MGI; MGI:2149330; Slc5a5. Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911. |
| Disease | Malignant neoplasms of the mouse mammary gland (NCIt: C21678) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: BALB/cfC3H. |
| Hierarchy | Parent: CVCL_0125 (4T1) |
| Sex of cell | Female |
| Category | Cancer cell line |
| Publications | PubMed=30674994; DOI=10.1038/s41417-019-0081-2; PMCID=PMC7170803 |
| Cross-references | |
| Cell line collections (Providers) | Imanis; CL066 |
| Encyclopedic resources | Wikidata; Q54603553 |
| Entry history | |
| Entry creation | 05-Mar-2018 |
| Last entry update | 10-Sep-2024 |
| Version number | 11 |