Cellosaurus 4T1-mNIS-Puro/Fluc-Neo (CVCL_QZ65)
Cell line name | 4T1-mNIS-Puro/Fluc-Neo |
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Synonyms | 4T1-Fluc-Neo/mNIS-Puro |
Accession | CVCL_QZ65 |
Resource Identification Initiative | To cite this cell line use: 4T1-mNIS-Puro/Fluc-Neo (RRID:CVCL_QZ65) |
Comments | Characteristics: Reporter cell line, transfected with both the LV-mNIS-PGK-Puro and LV-Fluc-P2A-Neo vectors. LV-mNIS-PGK-Puro encodes for mouse Slc5a5 under control of the spleen focus-forming virus (SFFV) promoter and the pac gene under the control of the mouse PGK promoter. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL066). Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG). Transfected with: MGI; MGI:2149330; Slc5a5. Transfected with: UniProtKB; P08659; Firefly luciferase. Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911. |
Disease | Malignant neoplasms of the mouse mammary gland (NCIt: C21678) |
Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: BALB/cfC3H. |
Hierarchy | Parent: CVCL_0125 (4T1) |
Sex of cell | Female |
Category | Cancer cell line |
Publications | PubMed=30674994; DOI=10.1038/s41417-019-0081-2 |
Cross-references | |
Cell line collections (Providers) | Imanis; CL066 |
Encyclopedic resources | Wikidata; Q54603553 |
Entry history | |
Entry creation | 05-Mar-2018 |
Last entry update | 02-May-2024 |
Version number | 10 |