Cellosaurus 4T1-mNIS-Neo/eGFP-Puro (CVCL_QZ62)
| Cell line name | 4T1-mNIS-Neo/eGFP-Puro |
|---|---|
| Accession | CVCL_QZ62 |
| Resource Identification Initiative | To cite this cell line use: 4T1-mNIS-Neo/eGFP-Puro (RRID:CVCL_QZ62) |
| Comments | Characteristics: Reporter cell line, transfected with both the LV-mNIS-P2A-Neo and LV-eGFP-PGKp-PuroR vectors. LV-mNIS-P2A-Neo encodes for mouse Slc5a5 linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGKp-PuroR encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the PuroR gene under the control of the mouse PGK promoter (Imanis=CL025). Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG). Genetic integration: Method=Transfection; Gene=MGI; MGI:2149330; Slc5a5. Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911. |
| Disease | Malignant neoplasms of the mouse mammary gland (NCIt: C21678) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: BALB/cfC3H. |
| Hierarchy | Parent: CVCL_0125 (4T1) |
| Sex of cell | Female |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Imanis; CL025 |
| Encyclopedic resources | Wikidata; Q54603546 |
| Entry history | |
| Entry creation | 05-Mar-2018 |
| Last entry update | 10-Sep-2024 |
| Version number | 10 |