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Cellosaurus GM09947 (CVCL_N126)

[Text version]
Cell line name GM09947
Synonyms GM9947; GM09947A; GM09947a; 9947A; GM17234
Accession CVCL_N126
Resource Identification Initiative To cite this cell line use: GM09947 (RRID:CVCL_N126)
Comments Part of: Genetic Testing Reference Material (GeT-RM) samples.
Part of: Human variation panel.
Registration: National Institute of Standards and Technology, Standard Reference Materials; SRM 2391b.
Registration: National Institute of Standards and Technology, Standard Reference Materials; SRM 2392.
Population: Caucasian.
Biotechnology: Used a source of control genomic and mitochondrial DNA for USA forensic testing.
Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
Omics: DNA methylation analysis.
Omics: Mitochondrial genome sequenced.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
HLA typing Source: PubMed=29959025
Class I
HLA-AA*02:01:01,02:01:01
HLA-BB*44:02:01,51:01:01
HLA-CC*02:02:02,05:01:01
Class II
HLA-DPDPA1*01:03:01,01:03:01
DPB1*03:01:01G,04:02:01G
HLA-DQDQA1*04:02,05:01:01
DQB1*02:01:01,04:02:01
HLA-DRDRB1*03:01:01,08:01:01
DRB3*02:02:01
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 31Y
Category Transformed cell line
STR profile Source(s): CelloPub=CLPUB00756; CelloPub=CLPUB00757; PubMed=14642717; PubMed=24266330

Markers:
AmelogeninX
CSF1PO10,12
D1S165618.3
D2S44110,14
D2S133819,23
D3S135814,15
D5S81811
D7S82010,11
D8S117913
D10S124813,15
D12S39118,20
D13S31711
D16S53911,12
D18S5115,19
D19S43314,15
D21S1130
D22S104511,14
DXS10124,26
F13A016,16
F13B8,10
FESFPS10,12
FGA23,24
LPL11,12
Penta D12
Penta E12,13
SE3319,29.2
TH018,9.3
TPOX8
vWA17,18

Run an STR similarity search on this cell line
Publications

CLPUB00447
Mulivor R.A., Suchy S.F.
1992/1993 catalog of cell lines. NIGMS human genetic mutant cell repository. 16th edition. October 1992.
(In misc. document) Institute for Medical Research (Camden, N.J.) NIH 92-2011; pp.1-918; National Institutes of Health; Bethesda; USA (1992)

PubMed=8530025; DOI=10.1006/geno.1995.1130
Fregeau C.J., Aubin R.A., Elliott J.C., Gill S.S., Fourney R.M.
Characterization of human lymphoid cell lines GM9947 and GM9948 as intra- and interlaboratory reference standards for DNA typing.
Genomics 28:184-197(1995)

PubMed=14642717; DOI=10.1016/j.forsciint.2003.09.002
Szibor R., Edelmann J., Hering S., Plate I., Wittig H., Roewer L., Wiegand P., Cali F., Romano V., Michael M.
Cell line DNA typing in forensic genetics -- the necessity of reliable standards.
Forensic Sci. Int. 138:37-43(2003)

PubMed=14583597; DOI=10.1152/physiolgenomics.00163.2003
Pastinen T., Sladek R., Gurd S., Sammak A., Ge B., Lepage P., Lavergne K., Villeneuve A., Gaudin T., Brandstrom H., Beck A., Verner A., Kingsley J., Harmsen E., Labuda D., Morgan K., Vohl M.-C., Naumova A.K., Sinnett D., Hudson T.J.
A survey of genetic and epigenetic variation affecting human gene expression.
Physiol. Genomics 16:184-193(2004)

PubMed=16260726; DOI=10.1073/pnas.0508390102; PMCID=PMC1283473
Harris S.L., Gil G., Robins H., Hu W.-W., Hirshfield K.M., Bond E., Bond G., Levine A.J.
Detection of functional single-nucleotide polymorphisms that affect apoptosis.
Proc. Natl. Acad. Sci. U.S.A. 102:16297-16302(2005)

CLPUB00756
National Institute of Standards and Technology
Certificate of analysis: Standard Reference Material 2391b.
(In technical document) NIST SRM 2391b; pp.1-8; NIST; Gaithersburg; USA (2008)

PubMed=20889555; DOI=10.2353/jmoldx.2010.100090; PMCID=PMC2962854
Pratt V.M., Zehnbauer B.A., Wilson J.A., Epstein-Baak R., Babic N., Bettinotti M.P., Buller-Burckle A.M., Butz K.G., Campbell M., Civalier C., El-Badry A., Farkas D.H., Lyon E., Mandal S., McKinney J., Muralidharan K., Noll L., Sander T., Shabbeer J., Smith C.-Y.H., Telatar M., Toji L.H., Vairavan A., Vance C., Weck K.E., Wu A.H.B., Yeo K.-T.J., Zeller M., Kalman L.V.
Characterization of 107 genomic DNA reference materials for CYP2D6, CYP2C19, CYP2C9, VKORC1, and UGT1A1: a GeT-RM and Association for Molecular Pathology collaborative project.
J. Mol. Diagn. 12:835-846(2010)

PubMed=24266330; DOI=10.1021/ac403137h
Geng T., Novak R., Mathies R.A.
Single-cell forensic short tandem repeat typing within microfluidic droplets.
Anal. Chem. 86:703-712(2014)

CLPUB00757
Promega Corporation
PowerPlex Fusion 6C system. Instructions for use of products DC2705 and DC2720.
(In technical document) Promega technical manual TMD045; pp.1-75; Promega Corp.; Madison; USA (2015)

PubMed=26621101; DOI=10.1016/j.jmoldx.2015.08.005; PMCID=PMC4695224
Pratt V.M., Everts R.E., Aggarwal P., Beyer B.N., Broeckel U., Epstein-Baak R., Hujsak P., Kornreich R., Liao J., Lorier R., Scott S.A., Smith C.-Y.H., Toji L.H., Turner A., Kalman L.V.
Characterization of 137 genomic DNA reference materials for 28 pharmacogenetic genes: a GeT-RM collaborative project.
J. Mol. Diagn. 18:109-123(2016)

PubMed=29959025; DOI=10.1016/j.jmoldx.2018.05.009; PMCID=PMC6939753
Bettinotti M.P., Ferriola D., Duke J.L., Mosbruger T.L., Tairis N., Jennings L., Kalman L.V., Monos D.S.
Characterization of 108 genomic DNA reference materials for 11 human leukocyte antigen loci: a GeT-RM collaborative project.
J. Mol. Diagn. 20:703-715(2018)

Cross-references
Cell line collections (Providers) Coriell; GM09947
Coriell; GM17234
Cell line databases/resources CLO; CLO_0014340
CLO; CLO_0029743
Encyclopedic resources Wikidata; Q54844133
Gene expression databases GEO; GSM569608
GEO; GSM596298
GEO; GSM596658
GEO; GSM924836
Entry history
Entry creation05-Nov-2013
Last entry update30-Jan-2024
Version number21