Cellosaurus cell line MLO-Y4 (CVCL

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Cellosaurus MLO-Y4 (CVCL_M098)

[Text version]

Cell line name

MLO-Y4

Synonyms

MLOY4; Murine Long bone Osteocyte-Y4

Accession

CVCL_M098

Resource Identification Initiative

To cite this cell line use: MLO-Y4 (RRID:CVCL_M098)

Comments

Group: Patented cell line.
Group: Space-flown cell line (cellonaut).
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12161.
Characteristics: Useful to study effects of fluid flow and substrate stretching on activation of osteocyte-specific signaling pathways (Kerafast=EKC002).
Doubling time: 15.8 hours (CelloPub=CLPUB00625).
Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P03070; SV40 large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Bone, long bone; UBERON=UBERON_0002495.
Cell type: Osteocyte; CL=CL_0000137.

Species of origin

Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: (C57BL/6J x BALB/cJ) F2 Bgla promoter T-Ag transgenic.

Hierarchy

Children:

CVCL_C8UU (MLO-Y4-Luc)

CVCL_E0Z7 (Ubigene MLO-Y4 Tnfsf11 KO)

Sex of cell

Sex unspecified

Age at sampling

14D

Category

Transformed cell line

STR profile

Source(s): Ubigene=YC-C037

Markers:

Mouse STR 4-2	20.3,21.3
Mouse STR 5-5	14
Mouse STR 6-4	18
Mouse STR 6-7	17
Mouse STR 12-1	17
Mouse STR 15-3	22.3
Mouse STR 18-3	17,18
Mouse STR X-1	25

Publications

PubMed=9421234; DOI=10.1359/jbmr.1997.12.12.2014
Kato Y., Windle J.J., Koop B.A., Mundy G.R., Bonewald L.F.
Establishment of an osteocyte-like cell line, MLO-Y4.
J. Bone Miner. Res. 12:2014-2023(1997)

PubMed=10084404; DOI=10.1007/s007740050066
Bonewald L.F.
Establishment and characterization of an osteocyte-like cell line, MLO-Y4.
J. Bone Miner. Metab. 17:61-65(1999)

Patent=US6358737
Bonewald L.F., Kato Y.
Osteocyte cell lines.
Patent number US6358737, 19-Mar-2002

PubMed=12412815; DOI=10.1359/jbmr.2002.17.11.2068
Zhao S., Zhang Y.K., Harris S., Ahuja S.S., Bonewald L.F.
MLO-Y4 osteocyte-like cells support osteoclast formation and activation.
J. Bone Miner. Res. 17:2068-2079(2002)

CLPUB00625
Lau P., Hellweg C.E., Kirchner S., Arenz A., Baumstark-Khan C., Horneck G.
Induction of DNA-strand breaks after X-irradiation in murine bone cells of various differentiation capacities.
(In conference) Abstracts of the 35th COSPAR scientific assembly; pp.1960-1960; Committee on Space Research; Paris; France (2004)

PubMed=16738320; DOI=10.1128/mcb.02120-05; PMCID=PMC1489126
Zhang K.-Q., Barragan-Adjemian C., Ye L., Kotha S., Dallas M., Lu Y.-B., Zhao S.-J., Harris M., Harris S.E., Feng J.Q., Bonewald L.F.
E11/gp38 selective expression in osteocytes: regulation by mechanical strain and role in dendrite elongation.
Mol. Cell. Biol. 26:4539-4552(2006)

PubMed=22130923; DOI=10.1007/978-1-61779-415-5_6
Rosser J., Bonewald L.F.
Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5.
Methods Mol. Biol. 816:67-81(2012)

Cross-references

Cell line collections (Providers)

Kerafast; EKC002
Ubigene; YC-C037

Cell line databases/resources

Lonza; 166

Anatomy/cell type resources

BTO; BTO:0005489

Encyclopedic resources

Wikidata; Q54905979

Experimental variables resources

EFO; EFO_0005394

Gene expression databases