Cellosaurus cell line CHO-K1 tsTM3 (CVCL

Cellosaurus CHO-K1 tsTM3 (CVCL_LH72)

Cross-references
Cell line name	CHO-K1 tsTM3
Synonyms	tsTM3
Accession	CVCL_LH72
Resource Identification Initiative	To cite this cell line use: CHO-K1 tsTM3 (RRID:CVCL_LH72)
Comments	Characteristics: Temperature sensitive. Exhibits chromosomal instability and cell-cycle arrest at S and G2 phases with decreased DNA synthesis at the non-permissive temperature of 39 Celsius. Transformant: ChEBI; CHEBI:21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Derived from site: In situ; Ovary; UBERON=UBERON_0000992. Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations	Mutation; UniProtKB; G3IBG3; Uba1; Simple; p.Met256Ile (c.768G>A); Zygosity=Unspecified (PubMed=24805847).
Species of origin	Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy	Parent: CVCL_0214 (CHO-K1)
Sex of cell	Female
Age at sampling	Adult
Category	Transformed cell line
Publications	PubMed=2237640; DOI=10.1007/BF01233196 Tsuji H., Matsudo Y., Tsuji S., Hanaoka F., Hyodo M., Hori T.-a. Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting chromosomal instability and reduced DNA synthesis at nonpermissive temperature. Somat. Cell Mol. Genet. 16:461-476(1990) PubMed=24805847; DOI=10.1371/journal.pone.0096666; PMCID=PMC4013028 Sugaya K., Ishihara Y., Inoue S., Tsuji H. Characterization of ubiquitin-activating enzyme Uba1 in the nucleus by its mammalian temperature-sensitive mutant. PLoS ONE 9:E96666-E96666(2014) PubMed=26059705; DOI=10.1111/gtc.12257 Sugaya K., Ishihara Y., Inoue S. Nuclear localization of ubiquitin-activating enzyme Uba1 is characterized in its mammalian temperature-sensitive mutant. Genes Cells 20:659-666(2015)
Encyclopedic resources	Wikidata; Q54973165
Entry history
Entry creation	15-Nov-2017
Last entry update	30-Jan-2024
Version number	8