Cellosaurus GeneBLAzer MR-UAS-bla HEK 293T (CVCL_LF47)
| Cell line name | GeneBLAzer MR-UAS-bla HEK 293T |
|---|---|
| Synonyms | MR-UAS-bla HEK 293T |
| Accession | CVCL_LF47 |
| Resource Identification Initiative | To cite this cell line use: GeneBLAzer MR-UAS-bla HEK 293T (RRID:CVCL_LF47) |
| Comments | Characteristics: Transfected with a plasmid containing a beta-lactamase reporter gene under control of an upstream activator sequence (UAS). Characteristics: Transfected with the ligand-binding domain (LBD) of NR3C2/MR fused to the DNA-binding domain (DBD) of GAL4. When an agonist binds to the LBD of the DBD-LBD fusion protein, the protein binds to the UAS, resulting in expression of the beta-lactamase. Genetic integration: Method=Transfection; Gene=HGNC; 7979; NR3C2 (Note=Ligand-binding domain). Genetic integration: Method=Transfection; Gene=UniProtKB; P62593; Escherichia coli beta-lactamase bla (Note=With p.Met1_Phe22del, p.Ala23Met, p.Val82Ile and p.Met184Val). Genetic integration: Method=Transfection; Gene=UniProtKB; P04386; Yeast GAL4 (Note=DNA-binding domain). Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA). Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_LF40 (GeneBLAzer UAS-bla HEK 293T) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Web pages | https://www.thermofisher.com/order/catalog/product/K1696 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q54835706 |
| Entry history | |
| Entry creation | 22-Aug-2017 |
| Last entry update | 10-Sep-2024 |
| Version number | 11 |