Cellosaurus NAKT-15 (CVCL_L910)
| Cell line name | NAKT-15 |
|---|---|
| Synonyms | NAKT15 |
| Accession | CVCL_L910 |
| Resource Identification Initiative | To cite this cell line use: NAKT-15 (RRID:CVCL_L910) |
| Comments | Characteristics: Secretes insulin in response to glucose stimulation and nonglucose secretagogues, and expresses proteins characteristic of beta-cells. Characteristics: Because it has been transduced by two constructs containing LoxP sites, transfection with the Cre recombinase eliminates both the SV40 T gene and TERT thus reverting the cells to a non-immortal phenotype. Characteristics: Transduced with two retroviral vectors: 1) SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by two loxP sites and 2) SSR#69 (MoMLV/LTR-LoxP-HygroR-HSV/TK-IRES-SV40T-LoxP-Neo-MoMLV/LTR) expressing HygroR, HSV-1 TK and SV40 T-antigen flanked by two loxP sites. Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT. Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transduction; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK. Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Pancreas, islets of Langerhans; UBERON=UBERON_0000006. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Sex of cell | Sex unspecified |
| Age at sampling | Age unspecified |
| Category | Transformed cell line |
| Publications | PubMed=16186810; DOI=10.1038/nbt1145 PubMed=16211061; DOI=10.1038/nbt1005-1231 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q54907375 |
| Entry history | |
| Entry creation | 06-May-2013 |
| Last entry update | 10-Apr-2025 |
| Version number | 15 |