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Cellosaurus PathHunter NCI-H460 CDC25A Degradation (CVCL_KZ69)

[Text version]
Cell line name PathHunter NCI-H460 CDC25A Degradation
Accession CVCL_KZ69
Resource Identification Initiative To cite this cell line use: PathHunter NCI-H460 CDC25A Degradation (RRID:CVCL_KZ69)
Comments Population: Caucasian.
Characteristics: PathHunter degradation cell lines express a target protein tagged with a beta-galactosidase fragment (Enzyme Donor=ED). Pathway activation results in the degradation of the target protein. The degraded protein is detected by adding to the cell lysis buffer another beta-galactosidase fragment (Enzyme Acceptor=EA). Complementation of the ED and EA enzyme fragments result into a catalytically active enzyme that generate a chemiluminescent signal.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:1725; CDC25A.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gln61His (c.183A>T); ClinVar=VCV000045117; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung large cell carcinoma (NCIt: C4450)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0459 (NCI-H460)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
Cross-references
Cell line collections (Providers) DiscoverX; 93-0776C12
Cell line databases/resources cancercelllines; CVCL_KZ69
Encyclopedic resources Wikidata; Q54938063
Entry history
Entry creation22-Aug-2017
Last entry update19-Dec-2024
Version number11