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Cellosaurus CHO dhfr(-) E1B-19K (CVCL_JZ94)

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Cell line name CHO dhfr(-) E1B-19K
Accession CVCL_JZ94
Resource Identification Initiative To cite this cell line use: CHO dhfr(-) E1B-19K (RRID:CVCL_JZ94)
Comments Group: Patented cell line.
Registration: International Depositary Authority, Korean Collection for Type Culture; KCTC 10143BP.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03246; Adenovirus 5 E1B 19 kDa protein.
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_1977 (CHO-DXB11)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

Patent=US20040014218
Lee G.M., Lee S.K.
DHFR-deficient CHO cell line transfected with an anti-apoptotic gene, method for preparation thereof, and method for producing target protein using the same.
Patent number US20040014218, 22-Jan-2004

Cross-references
Encyclopedic resources Wikidata; Q54812578
Entry history
Entry creation22-Aug-2017
Last entry update19-Dec-2024
Version number10