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Cellosaurus A-673 clone Asp114 (CVCL_JM58)

[Text version]
Cell line name A-673 clone Asp114
Synonyms A673 clone Asp114; ASP114; Asp114; A673/TR/shEF
Accession CVCL_JM58
Resource Identification Initiative To cite this cell line use: A-673 clone Asp114 (RRID:CVCL_JM58)
Comments Problematic cell line: Misclassified. Parent cell line (A-673) was originally thought to be a rhabdomyosarcoma cell line but is an Ewing sarcoma cell line.
Characteristics: Transfected with a construct harboring a doxycycline-inducible shRNA against the EWSR1-FLI1 fusion protein.
Knockout cell: Method=shRNA knockdown; HGNC; 3508; EWSR1 (EWSR1-FLI1 fusion).
Knockout cell: Method=shRNA knockdown; HGNC; 3749; FLI1 (EWSR1-FLI1 fusion).
Omics: Deep proteome analysis.
Omics: Transcriptome analysis by microarray.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Gene fusion; HGNC; 3508; EWSR1 + HGNC; 3749; FLI1; Name(s)=EWSR1-FLI1, EWS-FLI1; Note=Type 1 fusion (Ex7/Ex6) (from parent cell line).
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Ala119Glnfs*5 (c.353_354dupCA) (c.354_355insCA); Zygosity=Homozygous (from parent cell line).
Disease Ewing sarcoma (NCIt: C4817)
Ewing sarcoma (ORDO: Orphanet_319)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0080 (A-673)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line

PubMed=17438102; DOI=10.1158/1078-0432.CCR-06-1762
Carrillo J., Garcia-Aragoncillo E., Azorin D., Agra N., Sastre A., Gonzalez-Mediero I., Garcia-Miguel P., Pestana A., Gallego S., Segura D., Alonso J.
Cholecystokinin down-regulation by RNA interference impairs Ewing tumor growth.
Clin. Cancer Res. 13:2429-2440(2007)

PubMed=23940108; DOI=10.1101/gr.151340.112
Bilke S., Schwentner R., Yang F., Kauer M., Jug G., Walker R.L., Davis S., Zhu Y.J., Pineda M.A., Meltzer P.S., Kovar H.
Oncogenic ETS fusions deregulate E2F3 target genes in Ewing sarcoma and prostate cancer.
Genome Res. 23:1797-1809(2013)

PubMed=28062706; DOI=10.1158/1535-7163.MCT-16-0235
Radic-Sarikas B., Tsafou K.P., Emdal K.B., Papamarkou T., Huber K.V.M., Mutz C., Toretsky J.A., Bennett K.L., Olsen J.V., Brunak S., Kovar H., Superti-Furga G.
Combinatorial drug screening identifies Ewing sarcoma-specific sensitivities.
Mol. Cancer Ther. 16:88-101(2017)

Encyclopedic resources Wikidata; Q54606058
Gene expression databases GEO; GSE27524
Proteomic databases PRIDE; PXD002765
Entry history
Entry creation22-Aug-2017
Last entry update21-Mar-2023
Version number13