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Cellosaurus A-673 clone Asp114 (CVCL_JM58)

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Cell line name A-673 clone Asp114
Synonyms A673 clone Asp114; ASP114; Asp114; A673/TR/shEF
Accession CVCL_JM58
Resource Identification Initiative To cite this cell line use: A-673 clone Asp114 (RRID:CVCL_JM58)
Comments Problematic cell line: Misclassified. Parent cell line (A-673) was originally thought to be a rhabdomyosarcoma cell line but is an Ewing sarcoma cell line.
Population: Caucasian.
Characteristics: Transfected with a construct harboring a doxycycline-inducible shRNA against the EWSR1-FLI1 fusion protein.
Knockout cell: Method=shRNA knockdown; HGNC; HGNC:3508; EWSR1 (Note=EWSR1-FLI1 fusion).
Knockout cell: Method=shRNA knockdown; HGNC; HGNC:3749; FLI1 (Note=EWSR1-FLI1 fusion).
Omics: Proteomics.
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Muscle; UBERON=UBERON_0001630.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Gene fusion; HGNC; HGNC:3508; EWSR1 + HGNC; HGNC:3749; FLI1; Name(s)=EWSR1-FLI1, EWS-FLI1; Note=Type 1 fusion (Ex7/Ex6) (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Ala119Glnfs*5 (c.353_354dupCA) (c.354_355insCA); Zygosity=Homozygous (from parent cell line).
Disease Ewing sarcoma (NCIt: C4817)
Ewing sarcoma (ORDO: Orphanet_319)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0080 (A-673)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=17438102; DOI=10.1158/1078-0432.CCR-06-1762
Carrillo J., Garcia-Aragoncillo E., Azorin D., Agra N., Sastre A., Gonzalez-Mediero I., Garcia-Miguel P., Pestana A., Gallego S., Segura D., Alonso J.
Cholecystokinin down-regulation by RNA interference impairs Ewing tumor growth.
Clin. Cancer Res. 13:2429-2440(2007)

PubMed=23940108; DOI=10.1101/gr.151340.112; PMCID=PMC3814880
Bilke S., Schwentner R., Yang F., Kauer M., Jug G., Walker R.L., Davis S., Zhu Y.-L.J., Pineda M.A., Meltzer P.S., Kovar H.
Oncogenic ETS fusions deregulate E2F3 target genes in Ewing sarcoma and prostate cancer.
Genome Res. 23:1797-1809(2013)

PubMed=28062706; DOI=10.1158/1535-7163.MCT-16-0235
Radic-Sarikas B., Tsafou K.P., Emdal K.B., Papamarkou T., Huber K.V.M., Mutz C., Toretsky J.A., Bennett K.L., Olsen J.V., Brunak S., Kovar H., Superti-Furga G.
Combinatorial drug screening identifies Ewing sarcoma-specific sensitivities.
Mol. Cancer Ther. 16:88-101(2017)

Cross-references
Cell line databases/resources cancercelllines; CVCL_JM58
Encyclopedic resources Wikidata; Q54606058
Gene expression databases GEO; GSE27524
Proteomic databases PRIDE; PXD002765
Entry history
Entry creation22-Aug-2017
Last entry update10-Apr-2025
Version number19